In theory, cDNA analysis would be of enormous benefit in diagnostic medicine, since it obviates the need to examine many exons individually, at the same time providing the means to detect RNA splicing ...

Johns Hopkins researchers have developed a powerful new AI tool called Splam that can identify where splicing occurs in genes—an advance that could help scientists analyze genetic data with greater ...

A 760-kb YAC was constructed by homologous recombination in yeast, containing the genes located in the distal portion of the DMD gene. The YAC was introduced in mouse LA-9 cells by PEG-mediated cell ...

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...

Our cells have to generate a massive number of proteins, and there is a carefully orchestrated procedure for doing so. It starts when active genes are transcribed into RNA, and while that RNA ...

Although heart cells and skin cells contain identical instructions for creating proteins encoded in their DNA, they're able to fill such disparate niches because molecular machinery can cut out and ...

One idea has been that speckles are splicing factories. The splicing reaction would happen inside the speckle, and then the spliced product would leave. “What people found is that’s not what happens,” ...

Gene therapy (introducing genetic material into living cells to fix, replace, enhance, or block a faulty gene) is rapidly gaining traction as a strategy for the treatment of genetic diseases. The ...